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chroma spin te 400 columns  (TaKaRa)


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    Structured Review

    TaKaRa chroma spin te 400 columns
    Chroma Spin Te 400 Columns, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chroma+spin+400+columns/CHROMA+SPIN+%2BTE-400+Columns/10__3389_slash_av__2026__15087-58-21-24
    Average 94 stars, based on 168 article reviews
    chroma spin te 400 columns - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Fractionation:

    Article Title: High-Throughput Yeast Screening and Transcriptomic Integration Identify Salt-Tolerance Genes in Spartina alterniflora
    Article Snippet: The synthesized dsDNA was verified by agarose gel electrophoresis, treated to proteinase K, and digested with SfiI (New England Biolabs Inc., USA). .. Size fractionation was performed using CHROMA SPIN-400 columns (Clontech Laboratories Inc., USA). ..

    Extraction:

    Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
    Article Snippet: Second-strand synthesis was conducted in the presence of both primers using Advantage 2 Polymerase Mix (Clontech), under the following PCR conditions: 95°C for 20 s, followed by 22 cycles of 95°C for 5 s and 68°C for 6 min, concluding at 72°C for 10 min. .. Following proteinase K digestion and phenol:chloroform extraction, the amplified cDNAs were digested with Sfi I at 50°C for 2 h and size fractionated using CHROMA SPIN-400 columns (Clontech). .. Fractions containing cDNAs longer than 500 bp, as judged by 1% agarose gel electrophoresis, were pooled, ethanol precipitated, and ligated into λTripIEx2 (Clontech).

    Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae , after sugar feeding, blood feeding and Plasmodium berghei infection
    Article Snippet: .. Following proteinase K digestion and phenol:chloroform extraction, the amplified cDNAs were digested with 10 μl Sfi I (20 U/μl) at 50°C for 2 h and size fractionated using CHROMA SPIN-400 columns (Clontech). .. The first three to four fractions containing cDNAs longer than 500 bp were pooled, ethanol precipitated, and concentrated in 4.0 l nuclease free water (Gibco, UltraPure).

    Amplification:

    Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
    Article Snippet: Second-strand synthesis was conducted in the presence of both primers using Advantage 2 Polymerase Mix (Clontech), under the following PCR conditions: 95°C for 20 s, followed by 22 cycles of 95°C for 5 s and 68°C for 6 min, concluding at 72°C for 10 min. .. Following proteinase K digestion and phenol:chloroform extraction, the amplified cDNAs were digested with Sfi I at 50°C for 2 h and size fractionated using CHROMA SPIN-400 columns (Clontech). .. Fractions containing cDNAs longer than 500 bp, as judged by 1% agarose gel electrophoresis, were pooled, ethanol precipitated, and ligated into λTripIEx2 (Clontech).

    Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae , after sugar feeding, blood feeding and Plasmodium berghei infection
    Article Snippet: .. Following proteinase K digestion and phenol:chloroform extraction, the amplified cDNAs were digested with 10 μl Sfi I (20 U/μl) at 50°C for 2 h and size fractionated using CHROMA SPIN-400 columns (Clontech). .. The first three to four fractions containing cDNAs longer than 500 bp were pooled, ethanol precipitated, and concentrated in 4.0 l nuclease free water (Gibco, UltraPure).

    Article Title: The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus
    Article Snippet: .. Following the second round of amplification and PCR purification, the cDNA samples were double-digested with Sfi I and Mme I (40 and 26 units per 150 μl reaction, respectively). cDNA species <500 bp were then removed using Chroma Spin 400 columns (Clontech) which had been equilibrated with TM buffer following the manufacturer's protocol. .. It should be noted that the Chroma Spin column protocol suggested in the Clontech SMART cDNA kit is non-optimal, and that following the protocol provided with the separately purchased columns is less labor-intensive and produces a higher yield of size-selected cDNA.

    Purification:

    Article Title: Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
    Article Snippet: .. After that, cDNA was purified using Chroma Spin-400 columns (CLONTECH). cDNA libraries were prepared by directional cloning of purified cDNA into the pBluescript II SK vector (Addgene), according to the manufacturer's instructions, and screened on LB medium (Ap r -IPTG/x-gal). ..

    Article Title: The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus
    Article Snippet: .. Following the second round of amplification and PCR purification, the cDNA samples were double-digested with Sfi I and Mme I (40 and 26 units per 150 μl reaction, respectively). cDNA species <500 bp were then removed using Chroma Spin 400 columns (Clontech) which had been equilibrated with TM buffer following the manufacturer's protocol. .. It should be noted that the Chroma Spin column protocol suggested in the Clontech SMART cDNA kit is non-optimal, and that following the protocol provided with the separately purchased columns is less labor-intensive and produces a higher yield of size-selected cDNA.

    Cloning:

    Article Title: Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
    Article Snippet: .. After that, cDNA was purified using Chroma Spin-400 columns (CLONTECH). cDNA libraries were prepared by directional cloning of purified cDNA into the pBluescript II SK vector (Addgene), according to the manufacturer's instructions, and screened on LB medium (Ap r -IPTG/x-gal). ..

    Plasmid Preparation:

    Article Title: Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensis
    Article Snippet: .. After that, cDNA was purified using Chroma Spin-400 columns (CLONTECH). cDNA libraries were prepared by directional cloning of purified cDNA into the pBluescript II SK vector (Addgene), according to the manufacturer's instructions, and screened on LB medium (Ap r -IPTG/x-gal). ..

    Synthesized:

    Article Title: cDNA Library for Mining Functional Genes in Sedum alfredii Hance Related to Cadmium Tolerance and Characterization of the Roles of a Novel SaCTP2 Gene in Enhancing Cadmium Hyperaccumulation.
    Article Snippet: Subscriber access provided by TRINITY COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    cDNA Library Assay:

    Article Title: cDNA Library for Mining Functional Genes in Sedum alfredii Hance Related to Cadmium Tolerance and Characterization of the Roles of a Novel SaCTP2 Gene in Enhancing Cadmium Hyperaccumulation.
    Article Snippet: Subscriber access provided by TRINITY COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Agarose Gel Electrophoresis:

    Article Title: cDNA Library for Mining Functional Genes in Sedum alfredii Hance Related to Cadmium Tolerance and Characterization of the Roles of a Novel SaCTP2 Gene in Enhancing Cadmium Hyperaccumulation.
    Article Snippet: Subscriber access provided by TRINITY COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Polymerase Chain Reaction:

    Article Title: The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus
    Article Snippet: .. Following the second round of amplification and PCR purification, the cDNA samples were double-digested with Sfi I and Mme I (40 and 26 units per 150 μl reaction, respectively). cDNA species <500 bp were then removed using Chroma Spin 400 columns (Clontech) which had been equilibrated with TM buffer following the manufacturer's protocol. .. It should be noted that the Chroma Spin column protocol suggested in the Clontech SMART cDNA kit is non-optimal, and that following the protocol provided with the separately purchased columns is less labor-intensive and produces a higher yield of size-selected cDNA.



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